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. 2018 Oct 9;19(10):3087. doi: 10.3390/ijms19103087

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Treatment with apocynin for a week reduces seizure-induced neuronal cell death. Anti-neuron nuclear antigen (NeuN) staining was performed to identify live neurons. (A) A micrograph showing live neurons in the sham group, the seizure control group, and the apocynin treatment group. Apocynin treatment shows a substantially increased number of NeuN⁺ cells in the hippocampus of the seizure group. Scale bar = 100 μm. (B) The graph shows the average number of live neurons (NeuN⁺ cells) in the hippocampus after seizure compared to the apocynin treatment group. Data: mean ± standard error of the mean (S.E.M.), n = 5–6 from each group. * p < 0.05. CA3 is not statistically significant. Veh = Vehicle; Apo = Apocynin; CA1 and CA3 = Cornu Ammonis 1 and 3 of the hippocampus area; Sub = Subiculum.