Figure 6.

Membrane localization of WT mCherryTM immunostained with anti-mCherry antibody. COS-1 cells were transfected with pSARMXmCherryTM WT. After 48 h, the living cells were incubated with rabbit anti-mCherry antibody on ice and then fixed with 4% formaldehyde and immunostained with secondary anti-rabbit IgG antibody conjugated with Alexa Fluor^TM Plus 488. The left panel shows surface immune-staining of mCherry, the middle panel shows the inherent mCherry fluorescence, and the right panel shows these two images overlayed. Mock-transfected COS-1 cells were processed in the same way; no signal was detected. One focal plane acquired with a spinning disk confocal microscope (Andor) is showed. Magnification 600×; scale bar 20 µm.