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. 2018 Sep 30;19(10):2996. doi: 10.3390/ijms19102996

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell viability of A549 cells treated with HSP70/90 family inhibitors, (A,B) VER155008 (HSP70 family; GRP75 and HSP70), (C,D) HA15 (GRP78) and (E,F) NVP-BEP800 (HSP90 family; GRP94, HSP90 and trap-1) in human MSC-CM during hypoxic (0.5% O₂) exposure for 24 h. The viability of A549 cells was assessed via MTT assay. Data presented as mean ± SD; n = 3 (*** p < 0.001 vs. normoxia control; and # p < 0.05, ### p < 0.001 vs. hypoxia control and † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. BMSC-CM / vehicle or ADSC-CM / vehicle). The resulting data were statistically analysed using one-way ANOVA and Tukey’s multiple comparison test.

Cell viability of A549 cells treated with HSP70/90 family inhibitors, (A,B) VER155008 (HSP70 family; GRP75 and HSP70), (C,D) HA15 (GRP78) and (E,F) NVP-BEP800 (HSP90 family; GRP94, HSP90 and trap-1) in human MSC-CM during hypoxic (0.5% O₂) exposure for 24 h. The viability of A549 cells was assessed via MTT assay. Data presented as mean ± SD; n = 3 (*** p < 0.001 vs. normoxia control; and # p < 0.05, ### p < 0.001 vs. hypoxia control and † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. BMSC-CM / vehicle or ADSC-CM / vehicle). The resulting data were statistically analysed using one-way ANOVA and Tukey’s multiple comparison test.