Figure 5.

Inhibition of miR-204 expression prevents aldosterone-induced increase of calcium channel α1 subunits. Graphs show mRNA levels for L-type and T-type Ca²⁺ channel pore subunits—α1C, α1G, and α1H—in control cells (Ctrl), as well as in antagomiR-204 inhibitor (37.5 nM for 24 h)-transfected (Inh), antgomiR-204 inhibitor (37.5 nM for 24 h)-transfected + aldosterone (1 µM for 24 h)-stimulated (Inh+Aldo), luciferase siRNA (37.5 nM for 24 h)-transfected (Ctrl Inh), and luciferase siRNA (37.5 nM for 24 h)-transfected + aldosterone (1 µM for 24 h)-stimulated (Ctrl Inh + Aldo) cardiomyocytes. The bars and error bars indicate the means ± SEMs (n = 3). ** means p < 0.01 vs. control.