Skip to main content
. 2018 Sep 27;19(10):2941. doi: 10.3390/ijms19102941

Figure 7.

Figure 7

Aldosterone stimulation and miR-204 overexpression reduce REST-NRSF protein expression. (A) The pictures represent immunoblotting which shows the expression of REST-NRSF (upper panel) and GAPDH (lower panel) detected with specific antibodies. Cardiomyocytes were incubated for 24 h with either 1 µM aldosterone (Aldo) or ethanol (1 µL), or infected with miR-204 adenovirus (AdmiR204), or shLuc adenovirus (AdLuc) for the same period. The picture is representative of three experiments performed from three different cell preparations. (B) The bar graph shows the mRNA expression level of the transcriptional repressor NRSF determined by RT-qPCR in control, aldosterone (1 µM), miR204 adenovirus infected or shLuc adenovirus infected cardiomyocytes. The bars and error bars indicate the means + SEMs (n = 3).