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Cytokine production by splenocytes in STDME-fed mice after AOM/DSS-induced CAC. Splenocytes from control or STDME-fed CAC-induced mice were isolated and stimulated with plate-bound anti-CD3ε mAb for 48 h. (A–C) IFN-γ (A); IL-4 (B) and IL-10 (C) cytokines in culture supernatant were detected by ELISA. All data are presented as mean ± SEM and assed with a two-tailed Student’s t-test. * p < 0.05; ns = not significant; n = 10/group. STDME, selenoneine-containing tuna dark muscle extract.
