Figure 6.

Relative cell growth and transformation frequency in Bhas 42 cells treatment with selenoneine. (A) The cells were cultured for 4 days, following at final concentration range of 0–10 μM for 7 days. The cell growth was determined by crystal violet assay; (B) The cells were cultured for 24 h. For growth phase, 1 μg/mL MCA was treated for 3 days, followed treatment with 0.3 μM RA in 0.5% (v/v) DMSO or 1 uM selenoneine in 1% (v/v) UPW for 10 days as stationary phase. After removal of RA of selenoneine from media, the cells were cultured for next seven days to observe the promotion activity in cell transformation. Each experiment was performed three times. All data are presented as mean ± SD and assed with a two-tailed Student’s t-test. * p < 0.05; DMSO, dimethyl sulfoxide; MCA, 3-Methylcholanthrene; RA, retinoic acid; SN, selenoneine; UPW, ultra pure water.