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Vpu constrains GaLV Env envelope pseudotyping in a polyubiquitin-dependent manner. 293FT cells were transfected with Vpu sensitive GaLV Env Δ8 or insensitive GaLV Env Δ12, HIV-CMV-GFP and a DN ubiquitin (Ub-K0) expression construct. After 48 h, media was transferred to 293T mCAT-1 cells and infectivity was measured by flow cytometry 48 h later. Bars are mean ± SD for three experimental replicates.
