Figure 6.

The effects of 1-MT application on TRP metabolism and LPS-induced immune response were investigated in two experiments. In both experiments, 1-MT and its control MYR were repeatedly injected subcutaneously (s.c.) as indicated, aiming at maximum levels of 1-MT at the time of LPS or NaCl injections at 0 h according to the 1-MT kinetics. In experiment 1 (n = 47), blood samples were taken before and at 0, 1, 3, 6, 12 and 24 h after LPS/NaCl application and tissue was sampled after 6 h (n = 24) and after 24 h (n = 23). In experiment 2 (n = 48), blood samples were taken before and at 0, 6, 12 and 24 h after LPS/NaCl application and behaviour was observed in the period from 0 to 5 h. Both experiments were conducted in three replicates with 15 or 16 animals each.