Figure 3.

Osteoclastogenic differentiation of primary osteoclasts on different rough surfaces. Primary mouse macrophages were cultured on glass control and different rough titanium with M-CSF and RANKL for 4 days. (A) Osteoclasts were then stained with TRAP biochemical staining (n = 4). (B) Cell number on these surfaces was quantified by the DNA content (n = 4), and (C) their osteoclastogenic differentiation was determined by TRAP activity (n = 4). A significant difference was indicated by a, b, c d, and e. Groups with different letters mean significant difference, and groups sharing the same letter are not significantly different.