Figure 4.

Number, size, and cytoskeletal organization of RAW264.7-derived osteoclasts on different rough surfaces. RAW264.7-derived macrophages were cultured on glass control and different rough titanium with RANKL for 4 days. (A) Osteoclasts were then stained with DAPI (blue), Alexa Fluor 568 Phalloidin (red), and ELF 97 (green). (B) Perimeter of actin rings, (C) osteoclasts density, (D) nuclei number per osteoclast, and (E) osteoclast area on these different surfaces were determined to quantify the osteoclastogenic differentiation. Scale bar = 50 μm. A significant difference was indicated by a, b, c, and d. Groups with different letters mean significant difference and groups sharing the same letter are not significantly different.