Figure 6.

Anabolic effects and potential clastokines of conditioned medium of osteoclasts cultured on different rough surfaces. Mouse osteoprogenitor cells (MC3T3) and primary rat MSCs were cultured in the conditioned medium of osteoclasts cultured on glass control and different titanium rough surfaces and OM (50% v/v). (A) Mineralization nodules were stained with alizarin red and quantified with alizarin red (B) of mouse osteoprogenitor cells in a conditioned medium from RAW264.7-derived osteoclasts and (C) of primary rat MSCs in a conditioned medium from primary mouse osteoclasts. (D) Gene expression of six potential clastokines for osteoclasts on different surfaces and (E) protein concentration of two potential clastokines in a conditioned medium of RAW264.7-derived osteoclasts were quantified. A significant difference was indicated by a, b, c d, and e. Groups with different letters mean significant difference, and groups sharing the same letter are not significantly different.