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Stem-loop quantitative RT-PCR of miRNA accumulation in TPMVd-infected leaf tissue 4 w. p.i. relative to mock-inoculated controls to which a value of 1 was assigned. U6snRNA was used as an internal reference. Error bars indicate two times the value of SD for the corresponding data set. All fold changes were statistically significant from mock-inoculated controls when analyzed using a paired t-test (p < 0.05).
