Table 4.
Primers used for RT-PCR analysis. COX-2—cyclooxygenase 2; TNF-α—tumor necrosis factor alpha; iNOS—inducible nitric oxide synthase; NF-κB—nuclear factor kappa-light-chain-enhancer of activated B cells; GAPDH—glyceraldehyde 3-phosphate dehydrogenase; F—forward; R—reverse.
| Species | Gene | Primer Sequence | |
|---|---|---|---|
| Rat | COX-2 | F: TCGGAGGAGAAGTGGGTTTTAG | R: TTGATGGTGGCTGTCTTGGTAGG |
| TNF-α | F: GATGTGGAACTGGCAGAGGAG | R: CACGAGCAGGAATGAGAAGAG | |
| iNOS | F: TTGGAGCGAGTTGTGGATTGTT | R: TAGGTGAGGGCTTGCCTGAGTG | |
| NF- κ B | F: AACACTGCCGACCTCAAGAT | R: CATCGGCTTGAGAAAAGGAG | |
| GAPDH | F: TGAGGCCGGTGCTGAGTATGT | R: CAGTCTTCTGGGTGGCAGTGA | |