Figure 3.

Zinc inhibits androgen-stimulated AR transcriptional activity in AR(+) PCa cells. Cells were treated with either zinc or synthetic androgen, R1881, and luciferase assays were performed 24 h post-transfection. (A,B) The effect of zinc on ARE4-luc (A) and p61-luc (B) was tested in LNCaP cells in the absence and presence of R1881. (C,D) Forced expression of AR in the presence of zinc partly restored androgen-stimulated AR activity. (E) The effect of exogenous AR on PSA expression was demonstrated by Western blot analysis.