PG102 suppresses neutrophil chemotaxis in vitro and in vivo. (A) Chemotaxis assay of differentiated HL-60 (dHL-60) cells. (B) IHC staining for Ly6G in dorsal skin of control, vehicle, PG102 (100 mg/kg), Dex (1 mg/kg) treated mice. (C) Quantification of Ly6G+ cells per five random high power fields (400X). (D) Real-time qPCR analysis of mRNA expression levels of antimicrobial peptides and chemokines in dorsal skin of mice (n = 3–4). FBS, fetal bovine serum; Dex, dexamethasone; M5, mixture of 5 cytokines. The data are shown as the mean ± SEM. ####
p < 0.0001 versus Control group; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus vehicle group.