Table 1.
Target Gene | Primer Sequence (5′-3′) | |
---|---|---|
Forward | Reverse | |
hBD-2 | 5′-GGTGTTTTTGGTGGTATAGGC-3′ | 5′-AGGGCAAAAGACTGGATGACA-3′ |
hCXCL1 | 5′-AATCCTGCATCCCCCATA-3′ | 5′-TGTCTCTCTTTCCTCTTCTGTTCCT-3′ |
hCXCL5 | 5′-TGCGTTGCGTTTGTTTACAG-3′ | 5′-GAAAAGGGGCTTCTGGATCA-3′ |
hCXCL8 (IL-8) | 5′-ATGACTTCCAAGCTGGCCGTG-3′ | 5′-TTATGAATTCTCAGCCCTCTTCAAAAACTTCTC-3′ |
hHPRT | 5′-TATGGCGACCCGCAGCCCT-3′ | 5′-CATCTCGAGCAAGACGTTCAG-3′ |
hS100A8 | 5′-GGGAATTTCCATGCCGTCT-3′ | 5′-CCTTTTTCCTGATATACTGAGGAC-3′ |
hS100A9 | 5′-CAGCTGGAACGCAACATAGA-3′ | 5′-TCAGCTGCTTGTCTGCATTT-3′ |
mCXCL1 | 5′-CACCTCAAGAACATCCAGAGCT-3′ | 5′-CAAGCAGAACTGAACTACCATCG-3′ |
mDEFB4 | 5′-GCTTCAGTCATGAGGATCCAT-3′ | 5′-CTTGCTGGTTCTTCGTCTTTT-3′ |
mHPRT | 5′-CACAGGACTAGAACACCTGC-3′ | 5′-GCTGGTGAAAAGGACCTCT-3′ |
mCXCL2 | 5′-TTGCCTTGACCCTGAAGCCCCC-3′ | 5′-GGCACATCAGGTACGATCCAGGC-3′ |
mS100A8 | 5′-AAATCACCATGCCCTCTACAAG-3′ | 5′-CCCACTTTTATCACCATCGCAA-3′ |
mS100A9 | 5′-ATACTCTAGGAAGGAAGGACACC-3′ | 5′-TCCATGATGTCATTTATGAGGGC-3′ |
BD-2, beta-defensin 2; CXCL: C-X-C motif ligand; IL, interleukin, HPRT, hypoxanthine-guanine phosphoribosyltransferase; S100, S100 calcium binding protein; DEFB4, defensin beta 4; h, human; m, mouse; F, forward, R, reverse.