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. 2018 Sep 25;19(10):2915. doi: 10.3390/ijms19102915

Figure 2.

Figure 2

Incorporation of trifluridine (FTD) into DNA. SW620, HCT 116, and HT-29 cells were treated with 4.0 µM FTD and 10.0 µM regorafenib for 24 h, 4.0 µM FTD for 24 h followed by 10.0 µM regorafenib for 24 h, or regorafenib followed by FTD. Double-stranded DNA was extracted, and FTD incorporation was determined via liquid chromatography/tandem mass spectrometry (LC/MS/MS). Data are presented as the mean + SD (n = 3).