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. 2018 Sep 24;16(10):349. doi: 10.3390/md16100349

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE (12.5%) of the recombinant wild α-PsGal (lanes 1 and 2 before and after final purification stage, respectively) and its D451A and C494N mutants after the final stages of purification (lanes 3 and 4, respectively); molecular weight markers are shown in lane 5.