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. 2018 Sep 24;16(10):349. doi: 10.3390/md16100349

Figure 7.

Figure 7

Thin layer chromatography (TLC) profiles of the hydrolysis and transglycosylation products produced by recombinant α-PsGal. Lanes 1 and 8—α-PsGal with Gal-α(1→6)-Glcα,β at 20 °C and 8 °C, respectively. Lanes 2 and 4—α-PsGal with pNP-α-Gal at 20 °C and 8 °C, respectively. Lane 3—α-PsGal with mixture of Gal-α(1→6)-Glcα,β/pNP-α-Gal at 20 °C. Lane 5—D451A mutant with Gal-α(1→6)-Glcα,β/NaN3. Lane 6—D451A mutant with pNP-α-Gal/NaN3. Lane 7—D451A mutant with pNP-α-Gal. Blank mixtures: lane 9—Gal-α(1→6)-Glcα,β, lane 10—pNP-α-Gal, lane 11—Gal, and lane 12—Glc.