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. 2018 Sep 24;16(10):349. doi: 10.3390/md16100349

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mass spectrometry monitoring of the reaction of melibiose hydrolysis and transglycosylation catalyzed by wild α-PsGal in the buffered heavy-oxygen water at 20 °C. (a) Experimental time-dependent changes in integral intensity of matrix-assisted laser desorption ionization-mass spectroscopy (MALDI-MS) signals of the Hex₂ ions at 365 m/z (1) and 367 m/z (2), Hex at 203 m/z (3) and 205 m/z (4), and Hex₃ at 527 m/z (5) and 529 m/z; (b) time dependences of the MALDI-MS signals intensity of Hex₃ ions at 527 m/z (1) and 529 m/z (2) in an expanded scale.