Table 3.

Composition of reaction mixtures for monitoring of transglycosylation with various forms of the recombinant α-galactosidase from the marine bacterium Pseudoalteromonas sp. KMM 701.

Enzyme	Substrate	Acceptor	Substrate (mol)	Acceptor (mol)	V (mL)	U or mg	t (°C)	τ (h)
Wild	Gal-α(1→6)-Glcα,β	-	0.11	0	0.1	2.2	20	48
Wild	Gal-α(1→6)-Glcα,β	-	0.31	0	0.3	4.5	8	7 days
Wild	pNP-α-Gal	-	0.05	0	0.2	1.0	20	48
Wild	pNP-α-Gal	-	0.07	0	0.2	0.015	8	7 days
Wild	Gal-α(1→6)-Glcα,β	pNP-α-Gal	0.06	0.13	0.3	2.6	20	48
C494N	pNP-α-Gal	-	0.07	0	0.3	0.012	8	7 days
D451A	Gal-α(1→6)-Glcα,β	NaN3	0.06	0.3	0.1	(0.008)	20	75
D451A	pNP-α-Gal	NaN3	0.07	0.3	0.3	(0.024)	20	75
D451A	pNP-α-Gal	-	0.07	0	0.2	(0.016)	20	75