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TLC and matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) characteristics of reaction products of wild α-PsGal and its mutant C494N.
| Enzyme | Substrate | Product Number | TLC (Rf) | MALDI-MS (m/z) |
|---|---|---|---|---|
| Wild α-PsGal | Gal-α(1→6)-Glcα,β | 0.39 | 365 | |
| 1 | 0.52 | 203 | ||
| 2 | 0.55 | 203 | ||
| 3 | 0.35 | 365 | ||
| 4 | 0.35 | 365 | ||
| 5 | 0.26 | 527 | ||
| pNP-α-Gal | 0.83 | 324 | ||
| 1 | 0.52 | 203 | ||
| 6 | 0.61 | 486 | ||
| 4 | 0.34 | 365 | ||
| Mutant C494N | pNP-α-Gal | – | 324 | |
| 1 | – | 203 | ||
| 3 | – | 365 | ||
| 6 | – | 486 |
