Table 5.
Anomer signals of nuclear magnetic resonance (NMR) spectra of substrates and products of hydrolysis and transglycosylation catalyzed by wild α-PsGal and its mutant C494.
| Sugars | Product Number | δH (J in Hz) | δC | |||||
|---|---|---|---|---|---|---|---|---|
| A | B | A | B | |||||
| αH1 | αH1 | βH1 | αC1 | αC1 | βC1 | |||
| Gal-α(1→6)-Glcα,β | 5.00 (3.7) | 5.24 | 4.67 (7.8) | 98.2 | 92.2 | 96.1 | ||
| A | B | |||||||
| Galα,β | 1 | 5.26 (3.7) | 4.64 (7.9) | 97.7 | 93.6 | |||
| Glcα,β | 2 | 5.23 (3.7) | 4.58 (7.8) | 97.6 | 93.6 | |||
| Gal-α(1→6)-Galα,β | 3 | 4.98 (4.8) | 5.27 | 4.59 (7.9) | 97.7 | 99.8 | ||
| A | B | |||||||
| Gal-α(1→4)-Glcα,β | 4 | 5.22 (3.2) | N/D * | 102.2 | N/D | N/D | ||
| A | B | |||||||
| Gal-(1→4)-Gal-(1→6)-Glc | 5 | undetected | ||||||
| pNP-α-Gal | 5.85 (3.74) | 98.1 | ||||||
| Gal-α(1→6)-Gal-α-pNP | 6 | 4.82 (3.85) | 5.91 (3.82) | 98.8 | 97.6 | |||
| A | B | |||||||
| Gal-α(1→3)-Gal-α-pNP | 7 | 5.87 (3.6) | N/D | N/D | N/D | |||
| A | B | |||||||
* N/D = not defined.