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. 2018 Sep 27;19(10):2939. doi: 10.3390/ijms19102939

Figure 1.

Figure 1

Immunolabeling of olfactory marker protein (OMP) and signaling proteins in TRPM5-GFP and Skn-1a−/− mice. (A–D) Immunolabel of OMP (red) in mature OSNs. TRPM5 expression in TRPM5-MCs and TRPM5-expressing OSNs of a TRPM5-GFP mouse are shown in green (GFP+; A,B). Immunolabels of olfactory signaling protein CNGA2 (green in E–H) and ACIII (red in I–L), and G-protein Gγ13 (cyan in M–P) are shown. (A,E,I,M) Vehicle (water)-exposed TRPM5-GFP mice. (B,F,J,N) chemical-exposed TRPM5-GFP mice. (C,G,K,O) Vehicle (water)-exposed Skn-1a−/− mice. (D,H,L,P) chemical-exposed Skn-1a−/− mice. For each antibody, images were taken under the same exposure time and from similar septal regions of coronal main olfactory epithelium (MOE) sections processed under the same conditions. DAPI staining is shown in blue. Scale bar: 40 μm.