Figure 2.

Effect of luteolin treatment for 16 weeks on hepatic lipotoxicity markers and antioxidant enzyme activities, and Masson’s trichrome staining of hepatic and adipose tissues in C57BL/6J mice fed the high-fat diet. (A) Plasma levels of the hepatic lipotoxicity markers GOT and GPT and the levels of hepatic mitochondrial H₂O₂ and TBARS. (B) Activities of the hepatic antioxidant enzymes CAT, SOD, GPx, and GR. (C) Masson’s trichrome staining of transverse liver and epididymal sections (200× magnification). Data are shown as means ± SEM. ND vs HFD: * p < 0.05, ** p <0.01, *** p < 0.001. HFD vs LU: ^§ p < 0.05, ^§§ p < 0.01, ^§§§ p < 0.001.