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. 2018 Oct 4;10(10):543. doi: 10.3390/v10100543

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The mucosal immunity of piglets induced by the iPEDVPT-P96 virus (■), the PEDVPT-P96 virus (▲), or mock-inoculated (●) at 0 and 28 days post-inoculation (DPI) and at 14 days post-challenge (DPC), after re-challenge or challenge with the PEDVPT-P5 virus. Each group consisted of 5 piglets. The mean S/P ratios of fecal (A), and salivary (B) anti-PEDV spike IgA in each group at a given time point were generated from the original optical density (OD) values using an in-house PEDV-Spike based indirect ELISA. The error bars indicate standard deviation. The arrow indicated the time point (28 DPI or 0 DPC) of challenge with PEDVPT-P5 virus. Statistically significant differences between each group were examined using a two-way ANOVA by time. Significant differences from the mock group are labeled with asterisks (p < 0.05).