Figure 1.

Phylogenetic relationships, gene structure and conserved protein motifs in GAox genes from cucumber. (A) The phylogenetic tree was constructed based on the full-length protein sequences of 17 CsGAox, 16 AtGAox and 19 OsGAox proteins using MEGA 7.0 software. Cucumber, Arabidopsis and rice GAoxs were labeled by red, green and blue dots; (B) Exon-intron structure of CsGAox genes. Black lines indicate introns. The 2OG-FeII_Oxy and DIOX_N domain is highlighted by red and dark green boxes, respectively. The number indicates the phases of corresponding introns; (C) The motif composition of CsGAox proteins. The motifs, numbers 1–10, are displayed in different colored boxes. The sequence logos and E values for each motif are given in Figure S3.