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. 2018 Oct 12;19(10):3135. doi: 10.3390/ijms19103135

Figure 5.

Figure 5

Ectopic expression of CsGA20ox1 in Arabidopsis. (A) Arabidopsis transformants were confirmed by Polymerase chain reaction (PCR) using CaMV35S promoter specific forward primer together with CsGA20ox1 reverse primer; (B) Relative expression of CsGA20ox1 in Col-0 and three T3 generation transgenic lines. Total RNA was extracted from 10-day-old seedlings, then analyzed by semi-quantitative RT-PCR. The AtActin11 gene was used as an internal control; (C) The morphology of 10-day-old seedlings of Col-0 and three over-expression (OE) lines; (D,E) Lateral root number (D) and the primary root length (E) in 10-day-old wild type (Col-0) and three OE lines. Values are the means with SE (n = 8–10). Different letters indicate significant differences between means (ANOVA with Tukey’s HSD test, p < 0.05).