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. 2018 Sep 20;37(21):e100056. doi: 10.15252/embj.2018100056

Figure 6. Refined transport mechanism of ABC exporter McjD based on structural, biochemical, and biophysical data.

Figure 6

  • A, B
    Binding of ATP alone (futile cycle) is not coupled to an opening of the TMD that remains occluded. NBDs can close in the presence of ATP and do not require substrate for closure. On the other hand, the NBDs are more dynamic and they can sample a nearly closed dimer that is driven to full closure in the presence of ATP. Binding of both ATP and MccJ25 are coupled to open up the TMD, which reverts back to an occluded conformation upon substrate release. ATP hydrolysis and ADP release reset the transporter to an inward conformation for a new transport cycle.