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. 2018 Sep 12;37(21):e100087. doi: 10.15252/embj.2018100087

Figure 5. Tyrosine hydroxylase phosphorylation in humans experiencing severe stress.

Figure 5

  1. The LC contains secretagogin+ neurons in humans, as well. (2) depicts secretagogin‐labeled perikarya residing in a dense meshwork of local fibers, likely dendrites. (A1) Secretagogin co‐exists with TH in most neurons (solid arrowheads). Scale bars = 350 μm (A), 120 μm (A/2), and 12 μm (A1).
  2. Total TH levels and their phosphorylation at Ser31 in suicidal subjects relative to age‐matched controls [n = 6 (suicide) and n = 7 (controls)]. *P < 0.05. Representative Western blots are shown in Fig EV4A.
  3. Total and Ser31‐phosphorylated TH in acute heart failure (AHF) in human subjects. Representative Western blot. (C1) Data from n = 7 subjects with acute heart failure and n = 6 controls. *P < 0.05.
  4. Immunoprecipitation (IP) using an anti‐secretagogin antibody as bait revealed increased secretagogin (SCGN)‐Erk1 interaction in LC micropunches after AHF. nt IgG, non‐targeting IgG. Representative immunoblot is shown. Experiments were performed in triplicate.
Data information: Data are expressed as means ± s.e.m. and were analyzed by Student's t‐test (B, C).