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. 2018 Sep 12;37(21):e100087. doi: 10.15252/embj.2018100087

Figure EV5. Electrophysiological properties of layer 5 pyramidal cells in the mPFC of Scgn −/− vs. wild‐type mice (related to Fig 6).

Figure EV5

  1. Representative current‐clamp recordings showing significantly hyperpolarized resting membrane potentials of layer (L) 5 pyramidal cells in the mPFC of Scgn −/− mice immediately upon membrane rupture (see panel C for quantitative data).
  2. Spontaneous postsynaptic currents in Scgn −/− mice. Neurons were clamped at positive voltages to preferentially resolve excitatory postsynaptic currents. Note the markedly increased frequency of postsynaptic excitation in Scgn −/− mice.
  3. Quantitative demonstration of a negative shift in neuronal resting membrane potential in Scgn −/− pyramidal cells; **P < 0.01, n = 33 (control) vs. n = 18 (Scgn −/−).
  4. Action potential parameters for L5 pyramidal cells in Scgn −/− mice vs. wild‐type littermates. (D) While action potential threshold was significantly reduced, neither its amplitude (D1) nor its half‐width changed in Scgn −/− cells (D2). *P < 0.05, n = 23 (control) vs. n = 16 (Scgn −/−).
  5. Quantitative depiction of spontaneous postsynaptic currents (sPSCs) isolated as in panel (B). Note a tendency toward an increase in sPSC frequency in Scgn −/− cells. Each data point represents the median value (pA) of recording sweeps per cell. n = 8 (control) vs. n = 7 (Scgn −/−).
  6. Membrane depolarization upon superfusion with 10 μM norepinephrine. On average, Scgn −/− neurons produced larger responses than their wild‐type counterparts. *P < 0.05, n = 15 (control) vs. n = 13 (Scgn −/−).
  7. Afterdepolarization evoked by 2‐s stimulus in the presence of NE with each neuron pre‐clamped to −60 mV. n = 8 (control) vs. n = 7 (Scgn −/−).
  8. Tissue levels of dopamine (DA) and 3,4‐dihydroxyphenylacetic acid (DOPAC), its major metabolite, in the medial prefrontal and somatosensory cortices of wild‐type and Scgn −/− mice that optionally underwent acute formalin stress. The stress‐induced increase in tissue norepinephrine (NE) content in wild‐type but not Scgn −/− mice is shown in Fig 6E. Data are from n > 4 animals/group.
Data information: Results in panels (C, D, and F) were statistically analyzed by one‐way ANOVA.