Reagent/resource	Source	Identifier
Antibodies
β‐Actin	Sigma	Cat#A1978
Calbindin	Synaptic Systems	Cat#2014104
CNTF	R&D Systems	Cat#AF‐557‐NA
CNTF receptor	R&D Systems	Cat#AF‐559‐NA
CRH	Santa Cruz	Cat#SC‐1759
Enkephalin	Immunostar	Cat#20065
Erk1/2	Cell Signaling	Cat#9107
Phosphorylated Erk1/2	Cell Signaling	Cat#9101
GFAP	DAKO	Cat#Z0334
GRIA1	Alomone	AGP‐009
Neuropeptide Y	Immunostar	Cat#22940
Nestin	Millipore	MAB353
Secretagogin	Gift from L. Wagner	(rabbit)
Secretagogin	R&D Systems	Cat#AF4878
Synaptophysin	Synaptic Systems	Cat#101‐002
SMI301	BioLegend	Cat#801702
Tyrosine hydroxylase (TH)	Merck/Millipore	Cat#AB152
TH phosphorylated at Ser31	Sigma	Cat#SAB4300674
TH phosphorylated at Ser40	Sigma	Cat#T9573
VGLUT1	Synaptic Systems	Cat#135‐202
VGLUT2	Synaptic Systems	Cat#135‐404
Biotinylated Donkey Anti‐Rabbit IgG	Jackson ImmunoR.	Cat#711‐065‐152
Cy2‐conjugated Donkey Anti‐Goat IgG	Jackson ImmunoR.	Cat#705‐225‐003
Cy2‐conjugated Donkey Anti‐Rabbit IgG	Jackson ImmunoR.	Cat#711‐225‐152
Cy3‐conjugated Donkey Anti‐Goat IgG	Jackson ImmunoR.	Cat#705‐165‐003
Cy3‐conjugated Donkey Anti‐Rabbit IgG	Jackson ImmunoR.	Cat#711‐165‐152
Cy3‐conjugated Donkey Anti‐Guinea Pig IgG	Jackson ImmunoR.	Cat#706‐165‐148
Cy5‐conjugated Donkey Anti‐Rabbit IgG	Jackson ImmunoR.	Cat#711‐175‐152
Cy2‐Streptavidin	Jackson ImmunoR.	Cat#016‐220‐084
Normal Goat IgG	Santa Cruz	Cat#sc‐2028
Dynabeads Protein G	Invitrogen	Cat#10004D
Donkey Anti‐Rabbit HRP	Jackson ImmunoR.	Cat#711‐035‐152
Donkey Anti‐Mouse HRP	Jackson ImmunoR.	Cat#715‐035‐151
Donkey Anti‐Goat HRP	Jackson ImmunoR.	Cat#705‐035‐147
Chemicals, peptides, and recombinant proteins
L‐norepinephrine	Sigma	Cat#74480
Recombinant Ciliary Neurotrophic Factor	Sigma	Cat#C3710
Biotinylated Dextran Amine (BDA, 10,000 MW)	Invitrogen	Cat#D1956
Biotinylated Dextran Amine (BDA, 3,000 MW)	Molecular Probes	Cat#D7135
Clarity Western ECL Substrate	BIO‐RAD	Cat#170‐5060
Vectastain ABC Kit	Vector Labs	Cat#PK‐6101
DAB Peroxidase Substrate Kit	Vector Labs	Cat#SK‐4100
jetPRIME	Polyplus	Cat#114‐15
TSA Plus Fluorescein System Kit	PerkinElmer	NEL741001KT
Ketamine (Calypsol)	Richter	Cat#A6A066
Xylazine (Nerfasin)	Le Vet Pharma	Cat#15H044
Isoflurane (Forane®)	AbbVie GmbH	Cat#B506
Hoechst 33,342	Thermo Fisher	Cat#H1399
Critical commercial assays
CNTF Rat ELISA kit	Thermo Fisher Sci.	Cat#ERCNTF
Deposited data
Single‐cell RNA‐seq of mouse hypothalamus cells from control and acute formalin‐stressed animals	NCBI Gene Expression Omnibus	GSE74672
Experimental models: cell lines
INS‐1E	Gift from P. Maechler
Experimental models: organisms/strains
Crh‐Ires‐Cre::egfp mouse	Gift from J.S. Bains	Cat#012704
cfos‐CreERT2 mouse	JAX Labs	Cat#021882
ROSA26‐stop‐ZsGreen1 f/f mouse (Ai6)	JAX Labs	Cat#007906
Scgn −/− mice	MMRRC for authors	Malenczyk et al (2017)
Cntf −/− mice	A. Giordano	Masu et al (1993)
Scgn‐Cre mouse	G. Szabó	Developed for this study
AAV8‐EF1a‐double floxed‐hChR2(H134R)‐mCherry‐WPRE‐HGHpA	Addgene Viral Service	Cat#20297
AAV8‐hSyn‐DIO‐hM3D(Gq)‐mCherry	Addgene Viral Service	Cat#44361
AAV8‐hSyn‐DIO‐hM4D(Gi)‐mCherry	Addgene Viral Service	Cat#44362
AAV8‐hSyn‐DIO‐hM3D(Gq)‐mCherry	Addgene Viral Service	Cat#44361
pAAV‐hSyn‐DIO‐mCherry	Addgene Viral Service	Cat#50459
AAV‐hSyn1‐GCaMP6f‐P2A‐nls‐dTomato	Addgene Viral Service	Cat#51085
Sequence‐based reagents
Primers for CNTF	IDT	Ref#72797892‐3
Control siRNA	Santa Cruz	Cat#sc‐37007
SCGN siRNA	Santa Cruz	Cat#sc‐153255
Accell SMARTpool CNTF siRNA	GE Dharmacon	Cat#A‐091086
Accell SMARTpool SCGN siRNA	GE Dharmacon	Cat#A‐092169
Accell SMARTpool non‐targeting siRNA	GE Dharmacon	Cat#D‐001950‐01
Software and algorithms
Clampfit 10.0	Molecular Devices	Ver. pCLAMP‐10
MiniAnalysis	Synaptosoft	“MiniAnalysis”
SigmaPlot	Systat
Statistical Package for the Social Sciences 17.0	SPSS Inc
Image Lab 5.0	BIO‐RAD