Figure EV2. N‐terminal and C‐terminal tagged Upf1 enrich similar sets of specific proteins.

1. Estimation of the levels of overexpression for N‐terminal tagged Upf1 fragments, in comparison with chromosomally C‐terminal tagged protein. G6PDH was used as a loading control. Serial dilutions were used to test the ability of the immunoblot signal to estimate protein levels.
2. Average enrichment values for purifications done with chromosomally C‐terminal tagged Upf1 (x‐axis) and N‐terminally TAP‐tagged Upf1.