Table 1.
Summary of the effects of AthCV1 on four Aspergillus species.
| A. thermomutatus | A. fumigatus | A. niger | A. nidulans | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Property | Growth Temp | Virus-Free | AthCV1 Infected | Virus-Free | AthCV1 Infected | Virus-Free | AthCV1 Infected | Virus-Free | AthCV1 Infected |
| Conidia production (per plate) | 37 °C | 2.1 (± 0.36) × 105 | 2.0 (± 0.10) × 106 * | 3.8 (± 0.42) × 108 | 1.6 (± 0.12) × 107 * | 1.8 (± 0.12) × 108 | 2.0 (± 0.14) × 107 * | 2.2 (± 0.62) × 108 | 9.5 (± 0.79) × 107 * |
| 20 °C | 2.4 (± 0.36) × 106 | 2.1 (± 0.24) × 105 * | 1.5 (± 0.21) × 108 | 3.6 (± 0.31) × 107 * | 2.2 (± 0.26) × 108 | 1.9 (± 0.24) × 108 | 1.4 (± 0.16) × 108 | 1.4 (± 0.12) × 108 | |
| Ascospore production (per plate) | 37 °C | 0 | 7.2 (± 0.73) × 105 * | 0 | 0 | 0 | 0 | 1.1 (± 0.10) × 106 | 2.1 (± 0.42) × 106 * |
| 20 °C | 0 | 3.6 (± 0.37) × 105 * | 0 | 0 | 0 | 0 | 2.9 (± 0.22) × 105 | 8.0 (± 0.55) × 105 * | |
| Sclerotia production (per plate) | 37 °C | 0 | 0 | 0 | 0 | 0 | 54 (± 4.2) * | 0 | 0 |
| 20 °C | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| Growth (mm) 1 | 37 °C | 74 (± 0.32) | 75 (± 1.00) | 72 (± 0.37) | 73 (± 1.00) | 77 (± 0.20) | 67 (± 0.68) * | 76 (± 0.49) | 75 (± 0.71) |
| 20 °C | 76 (± 0.32) | 75 (± 0.32) | 67 (± 0.32) | 72 (± 0.20) * | 77 (± 0.60) | 67 (± 0.74) | 33 (± 0.45) | 30 (± 0.66) * | |
| Fungal Biomass 2 (g dry wt) | 37 °C | 1.25 (± 0.012) | 1.28 (± 0.009) | 1.30 (± 0.008) | 1.23 (± 0.008) * | 1.25 (± 0.023) | 1.30 (± 0.007) | 1.36 (± 0.021) | 1.12 (± 0.005) * |
| 20 °C | 1.13 (± 0.011) | 1.13 (± 0.009) | 1.15 (± 0.011) | 1.13 (± 0.007) | 1.09 (± 0.004) | 1.12 (± 0.003) * | 1.12 (± 0.016) | 1.12 (± 0.010) | |
| Sector formation | 37 °C | Creamy, rough ascospore-rich sectors | Clear, elongated sectors in the grey mycelium | Conidia-free sectors with no pigmentation | Ascospore-rich sectors in the green mycelium | ||||
| 20 °C | Creamy, rough ascospore-rich sectors | Clear, elongated sectors in the grey mycelium | Conidia-free sectors with no pigmentation | No sector formation | |||||
| Pigment change | 37 °C | Creamy sectors | Sectors lack pigmentation | Sectors lack pigmentation | No change | ||||
| 20 °C | Creamy sectors | Sectors lack pigmentation | Sectors lack pigmentation | No change | |||||
Data analyzed by independent samples t-test using SPSS version 21, SEs in brackets. * = significant difference (p < 0.05) between virus-free and virus infected treatments.1 Since growth was measured to the edge of the petri dish the growth period differed for the different species: at 37 °C A. thermomutatus = 6 days, A. fumigatus = 6 days, A. niger = 13 days, A. nidulans = 13 days; at 20 °C A. thermomutatus = 15 days, A. fumigatus = 24 days, A. niger = 29 days, A. nidulans = 24 days. 2 Growth in liquid culture was measured after 4 days at 37 °C and 15 days at 20 °C.