Table 5.

Ingenuity toxicity list secondary to keratinocytes treatment with 20,23(OH)₂D3 in humans for 6 h. Nuclear receptors are marked in bold.

Ingenuity Toxicity Lists	p-Value	Overlap (%)	Downregulated	No Change	Upregulated	No Overlap with Dataset
Cytochrome P450 Panel—Substrate is a Vitamin (Human)	0.007244	16.7	2/6 (33%)	0/6 (0%)	4/6 (67%)	0/6 (0%)
Aryl Hydrocarbon Receptor Signaling	0.016218	1.24	71/161 (44%)	0/161 (0%)	75/161 (47%)	15/161 (9%)
Cytochrome P450 Panel—Substrate is a Sterol (Human)	0.016982	7.14	5/14 (36%)	0/14 (0%)	9/14 (64%)	0/14 (0%)
Cytochrome P450 Panel—Substrate is a Xenobiotic (Human)	0.021878	5.56	7/18 (39%)	0/18 (0%)	9/18 (50%)	2/18 (11%)
Nongenotoxic Hepatocarcinogenicity Biomarker Panel	0.026303	4.55	12/22 (55%)	0/22 (0%)	9/22 (41%)	1/22 (5%)
Cytochrome P450 Panel—Substrate is a Xenobiotic (Mouse)	0.0302	4	5/25 (20%)	0/25 (0%)	7/25 (28%)	13/25 (52%)
Cytochrome P450 Panel—Substrate is a Xenobiotic (Rat)	0.030903	3.85	5/26 (19%)	0/26 (0%)	7/26 (27%)	14/26 (54%)
Xenobiotic Metabolism Signaling	0.063096	0.595	155/336 (46%)	0/336 (0%)	139/336 (41%)	42/336 (13%)
VDR/RXR Activation	0.091201	1.28	34/78 (44%)	0/78 (0%)	43/78 (55%)	1/78 (1%)
TR/RXR Activation	0.097724	1.18	39/85 (46%)	0/85 (0%)	46/85 (54%)	0/85 (0%)
Hepatic Fibrosis	0.114025	1.01	49/99 (49%)	0/99 (0%)	47/99 (47%)	3/99 (3%)
Increases Liver Hyperplasia/ Hyperproliferation	0.116145	0.99	39/101 (39%)	0/101 (0%)	52/101 (51%)	10/101 (10%)
Renal Necrosis/Cell Death	0.12388	0.399	224/501 (45%)	1/501 (0%)	258/501 (51%)	18/501 (4%)
Fatty Acid Metabolism	0.133352	0.855	57/117 (49%)	0/117 (0%)	39/117 (33%)	21/117 (18%)
Cardiac Fibrosis	0.209894	0.521	89/192 (46%)	0/192 (0%)	87/192 (45%)	16/192 (8%)
Liver Proliferation	0.24322	0.441	98/227 (43%)	0/227 (0%)	118/227 (52%)	11/227 (5%)