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. 2018 Oct 5;19(10):3035. doi: 10.3390/ijms19103035
AAV Adeno-associated virus
CRISPR/Cas9 Clustered regularly-interspaced short palindromic repeats/CRISPR associated protein 9
CDM Chemically defined medium
DSB Double strand break
DE Definitive endoderm
EMCV-IRES Encephalomyocarditis virus internal ribosomal entry site
FIX Coagulation factor IX
FRT Flippase recognition target
HDR Homology directed recombination
HPLCs Hepatic progenitor like cells
ICG Indocyanine green
LHA Long homologous arm
mESCs Mouse embryonic stem cells
MH Maturated hepatocyte
ORF Open reading frame
PAS staining Periodic acid-Schiff’s staining
rDNA Ribosomal DNA
SCID Severe combined immune deficiency
SHA Short homologous arm
SSA Single strand annealing