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RT-PCR and Western blot assays for validating DERs and DEGs. (A) Stem-loop PCR assay for ten differentially co-expressed DERs. (B) Real-time PCR assay for ten differentially co-expressed DEGs. (C) Western blot assays for four highly co-expressed proteins in the three developmental stages. (D) The statistical graph for the four highly co-expressed proteins.
