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. 2018 Oct 9;19(10):3083. doi: 10.3390/ijms19103083

Figure 6.

Figure 6

The crucial role of AURKA of yak testis in development and gametogenesis. (A) Validating AURKA as a target of miR-574 using a dual luciferase reporter assay. (B,C) Validating STAG3 as a target of miR-21-3p and miR-2320-3p using a dual luciferase reporter assay. (D) The crucial DEGs of oocyte meiosis were chosen for evaluating the role of AURKA in different tissues by RT-PCR. (E) The crucial DEGs were detected in testis samples of different development stages by RT-PCR. (F) The relative expression level of these DEGs was gradually upregulated with testis development. (G) H&E staining of testis samples at different developmental stages. (H,I) Immunohistochemical stain assay for expression and location of AURKA and STAG3 in testis samples in the different development stages. (J) Western blot assay for detecting the expression level of AURKA and STAG3 in testis samples in different developmental stages. (K,L) Relative protein expression level of AURKA and STAG3.