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. 2018 Sep 20;16(10):344. doi: 10.3390/md16100344

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Ultrafiltration (UF) and diafiltration (DF) progress for samples obtained from NaOH (1 h and 24 h of hydrolysis) and NaOH-EtOH treatment. Top: concentration of retained protein (ο) and CS (●) in linear relation with the factor of volumetric concentration (fc) depicting experimental data (points) and theoretical profiles corresponding to a fully-retained solute (discontinuous line). Bottom: progress of protein (ο) and CS (●) retention with the increase of diavolume from DF step (D). Error bars are the confidence intervals (α = 0.05; n = 2).