Table 3.

Polynomial equations modelling pH and T effects on alcalase hydrolysis of G. melastomus cartilage. Adjusted determination coefficients ($R_{\mathrm{adj}}^2$) and optimal values of T and pH (T_opt and pH_opt) that maximized the dependent variables are also shown.

Second Order Equations	${\mathit{R}}_{\mathbf{adj}}^{\mathbf{2}}$	Topt (°C)	pH opt
Hm (%) = 22.02 − 5.18 T − 4.82 pH − 5.56 T pH − 4.26 T2 − 4.44 pH2	0.801	47.5	7.61
CS (g/L) = 5.25 − 0.80 T − 1.36 pH − 1.20 T pH − 0.80 T2 − 1.16 pH2	0.796	53.3	7.25
Ip (%) = 85.06 − 11.81 T − 23.06 pH − 22.76 T pH − 10.59 T2 − 20.02 pH2	0.890	57.8	7.06