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. 2018 Oct 2;19(10):3006. doi: 10.3390/ijms19103006

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoblot analyses of thylakoid proteins obtained from Z16, H89, CN19, and XZ31. Immunoblotting of thylakoid proteins were done using specific antibodies against representative photosystem I (PSI) (Lhca1, Lhca2, Lhca3, Lhca4, PsaD) and photosystem II (PSII) proteins (D1, D2, CP43, Lhcb1, Lhcb2, Lhcb3, Lhcb4, Lhcb5, Lhcb6) (A). Proteins corresponding to 0.25, 0.5, and 1 µg Chl (1/4, 1/2, and 1, respectively) were loaded. The SDS-PAGE results after Coomassie blue staining (CBS) were presented in the bottom panel (B). Loading was according to equal amount of total Chl (1 or 0.5 µg of Chl). Z16, H89, CN19, and XZ31 present accessions of Triticum monococcum (W2n), Triticum dicoccum (W4n), Triticum aestivum (W6n), and octaploid Triticale (T8n), respectively.