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. 2018 Sep 30;19(10):2997. doi: 10.3390/ijms19102997

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of lentiviral vector expressing NY CaN sensor in astrocytes. (A) A scheme of lentiviral vector expressing ΔNFAT-EYFP (NY) CaN sensor. (B) Hippocampal astrocytes were transduced with NY CaN sensor and then stimulated with 2 μM ionomycin. Nuclear translocation of NY in astrocyte was observed after 10 min of incubation. A representative astrocyte is shown from three independent preparations. Bar, 10 μm. (C) Quantification of nuclear-to-cytosol ratio (Nuc/Cyt ratio) of NY in the green channel. CaN was considered inactive with Nuc/Cyt ratio <1, while Nuc/Cyt ratio >1 indicates CaN activation.