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cLTP-induced NY nuclear translocation in astrocytes is CaN-dependent. (A) Scheme depicting cLTP induction protocol with consequent readouts. (B) Application of cLTP protocol to mixed neuron-astroglial hippocampal primary cultures induces robust NY translocation into the astrocyte nucleus at 15 min after cLTP induction phase. Note the complete inhibition of NY translocation when CaN inhibitor, FK506, was added during the cLTP development phase. Bar, 30 μm.
