Table 3.
Ranking of the expression of candidate HKG under acid pH culture conditions (pH 6.5).
| Gene | NormFinder | GeNorm | BestKeeper | ΔCt | Coefficient of Variation | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stability Value | Rank | M Value | Rank | ST.DEV [+/− CP] | Rank | ST.DEV | Rank | CV | Rank | |
| YWHAZ | 0.265 | 1 | 0.518 | 1 | 0.776 | 4 | 1.076 | 1 | 0.040 | 2 |
| RPL13a | 0.389 | 2 | 0.756 | 5 | 0.924 | 6 | 1.203 | 5 | 0.052 | 6 |
| PPIA | 0.398 | 3 | 0.682 | 4 | 0.969 | 7 | 1.164 | 4 | 0.058 | 9 |
| 18S rRNA | 0.401 | 4 | 0.518 | 1 | 0.604 | 2 | 1.144 | 3 | 0.084 | 14 |
| GUSB | 0.431 | 5 | 0.802 | 6 | 1.166 | 9 | 1.142 | 2 | 0.047 | 4 |
| ACTB | 0.461 | 6 | 0.861 | 8 | 0.872 | 5 | 1.330 | 10 | 0.054 | 7 |
| HMBS | 0.471 | 7 | 0.826 | 7 | 1.242 | 10 | 1.205 | 6 | 0.052 | 5 |
| GAPDH | 0.519 | 8 | 0.589 | 3 | 0.537 | 1 | 1.260 | 7 | 0.034 | 1 |
| PGK1 | 0.533 | 9 | 0.991 | 11 | 0.988 | 8 | 1.276 | 8 | 0.062 | 10 |
| HPRT1 | 0.570 | 10 | 0.911 | 9 | 1.397 | 13 | 1.287 | 9 | 0.065 | 11 |
| TUBB | 0.578 | 11 | 0.953 | 10 | 1.345 | 12 | 1.332 | 11 | 0.071 | 12 |
| SDHA | 0.655 | 12 | 1.085 | 13 | 1.338 | 11 | 1.461 | 12 | 0.074 | 13 |
| B2M | 0.697 | 13 | 1.039 | 12 | 0.680 | 3 | 1.467 | 13 | 0.044 | 3 |
| G6PD | 0.874 | 14 | 1.162 | 14 | 1.493 | 14 | 1.757 | 14 | 0.058 | 8 |
| TBP | 1.205 | 15 | 1.406 | 15 | 2.223 | 15 | 2.991 | 15 | 0.114 | 15 |