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. 2018 Sep 22;19(10):2886. doi: 10.3390/ijms19102886

Table 3.

Serum levels of soluble mediators and their associations with levels of immunocompetent and CD34+ cells in peripheral blood and stem cell grafts. We investigated the levels of six different immunocompetent cell subsets for 20 healthy stem cell donors. Serum levels and levels of circulating cells were determined after four days of G-CSF therapy before apheresis; graft composition was analyzed for the leukapheresis on day 4. For immunocompetent cells the results are presented as the cell number × 109/L in peripheral blood/grafts; for CD34+ cells the levels are presented as the number × 103/mL in peripheral blood and × 109/L in the grafts. Correlation coefficients (Spearman’s rho) between serum levels of IL-6 family cytokines/receptors/CRP and immunocompetent cell subsets in the graft and peripheral blood are also presented. Significant correlations are highlighted in bold (* p-value between 0.05 and 0.01, ** p-value below 0.01).

The Peripheral Blood Levels of Immunocompetent Cell Subsets
Leukocyte subset Peripheral Blood Level 1 IL-6 sIL-6R sgp130 IL-31 OSM CNTF CRP
T cells, total (CD3+) 3.31 (1.29–4.17) −0.042 −0.508 −0.697 ** 0.244 −0.511 0.654 * 0.156
CD4+ T cells 2.54 (0.92–3.47) 0.046 −0.582 * −0.609 * 0.354 −0.495 0.427 0.229
CD8+ T cells 0.60 (0.24–1.08) −0.135 −0.205 −0.557 * 0.104 −0.275 0.555 * 0.097
B cells (CD19+) 0.41 (0.21–1.77) 0.289 −0.310 −0.719 ** 0.525 −0.423 0.507 0.384
NK-cells (CD3− CD56+) 0.30 (0.07–0.77) −0.449 0.165 0.181 −0.020 0.366 −0.074 −0.249
Total monocytes 2.4 (0.90–3.9) −0.065 −0.164 −0.296 0.276 −0.046 0.362 0.210
CD34+ cells 40.2 (16.7–148) −0.21 −0.32 −0.54 * 0.47 0.37 0.33 0.045
The Graft Composition of Immunocompetent Cell Subsets
Leukocyte subset Graft Level 1 IL-6 sIL-6R sgp130 IL-31 OSM CNTF CRP
T cells, total (CD3+) 22.78 (8.41–42.81) −0.088 0.328 0.294 0.097 −0.074 −0.358 −0.539 *
CD4+ T cells 17.55 (6.02–31.66) 0.073 0.459 0.516 −0.162 −0.196 −0.176 −0.444
CD8+ T cells 4.64 (1.30–9.74) −0.068 0.336 0.204 0.087 −0.007 0.268 −0.592 *
B cells (CD19+) 3.63 (0.00–12.76) 0.534 * 0.363 0.169 0.184 0.385 0.277 −0.622 *
NK-cells (CD3− CD56+) 1.79 (0.40–5.50) −0.121 0.253 0.433 0.315 0.415 0.121 −0.407
Total monocytes 12.91 (1.93–25.23) −0.248 0.071 0.100 0.248 0.324 −0.054 −0.256
CD34+ cells 0.43 (0.085–201) −0.026 0.319 0.125 −0.258 −0.088 0.400 −0.009

1 Peripheral blood levels: The vertical column presents the cell subset, the horizontal line the serum soluble mediator.