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. 2018 Oct 12;19(10):3129. doi: 10.3390/ijms19103129

Figure 5.

Figure 5

Adeno-associated virus (AAV) transduction and hCycB silencing in printed mature HepaRG cells in alginate/gelatin bioinks containing 1 mg/mL hECM. (A) Analysis of AAV2.6 vector transduction and distribution within mature HepaRG cell-laden 3D alginate/gelatin constructs, determined by fluorescence microscopy. Comparison of constructs with and without 1 mg/mL hECM seven days after printing. Nuclei were visualized by Hoechst staining (blue); the green fluorescence represents green fluorescent protein (GFP) expression of AAV vectors. Scale bar: 200 µm; (B) Analysis of shRNA-mediated hCycB RNA knockdown within the mature HepaRG cell-laden 3D alginate/gelatin constructs, determined by reverse transcription polymerase chain reaction (RT-qPCR). Comparison of constructs with and without 1 mg/mL hECM seven days after transduction. An shRNA control was used for normalization to assess the knockdown. Results are shown as mean ± SEM of three independent experiments. *** p ≤ 0.001.