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. 2018 Oct 1;16(10):359. doi: 10.3390/md16100359

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression and purification analysis of PsLeuDH. Lane 1: molecular weight standard marker; Lane 2: crude extract from the BL21/pET-28a (+); Lane 3: crude extract from the BL21/pET-28a (+)-PsLeuDH with IPTG induction; Lane 4: rPsLeuDH eluted with 50 mM imidazole; Lane 5: rPsLeuDH eluted with 100 mM imidazole.