Table 2.
Effects of different reagents on the activity of rPsLeuDH.
| Reagent | Concentration | Relative Activity (%) | Reagent | Concentration | Relative Activity (%) |
|---|---|---|---|---|---|
| None | 100 ± 0.0 | ||||
| KCl | 1 mM | 99.7 ± 1.6 | KCl | 5 mM | 40.0 ± 1.9 |
| CoCl2 | 1 mM | 90.1 ± 1.7 | CoCl2 | 5 mM | 70.0 ± 2.0 |
| MgCl2 | 1 mM | 87.9 ± 0.8 | MgCl2 | 5 mM | 65.8 ± 1.2 |
| CaCl2 | 1 mM | 87.9 ± 0.4 | CaCl2 | 5 mM | 68.1 ± 0.9 |
| ZnCl2 | 1 mM | 80.0 ± 2.5 | ZnCl2 | 5 mM | 72.2 ± 2.0 |
| FeCl2 | 1 mM | 75.1 ± 2.2 | FeCl2 | 5 mM | 62.4 ± 1.7 |
| CuCl2 | 1 mM | 61.0 ± 2.2 | CuCl2 | 5 mM | 41.0 ± 1.5 |
| HgCl2 | 1 mM | 29.2 ± 0.3 | HgCl2 | 5 mM | 12.3 ± 1.9 |
| CrCl2 | 1 mM | 13.3 ± 0.3 | CrCl2 | 5 mM | 5.8 ± 2.9 |
| CdCl2 | 1 mM | 7.6 ± 0.5 | CdCl2 | 5 mM | 0.0 ± 0.0 |
| Pb(NO3)2 | 1 mM | 0.0 ± 0.0 | Pb(NO3)2 | 5 mM | 0.0 ± 0.0 |
| BaCl2 | 1 mM | 0.0 ± 0.0 | BaCl2 | 5 mM | 0.0 ± 0.0 |
| EDTA | 1 mM | 91.8 ± 2.7 | EDTA | 5 mM | 84.2 ± 2.1 |
| Thiourea | 1 mM | 51.5 ± 4.0 | Thiourea | 5 mM | 34.3 ± 2.6 |
| Triton X-100 | 0.2% | 102.7 ± 1.4 | Ethanol | 25% | 67.5 ± 1.4 |