Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 30;19(10):2990. doi: 10.3390/ijms19102990

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

OPNa, osteocalcin, and collagen type I transcript levels and calcification deposits in TPC-1 cell line. (A) OPNa, osteocalcin, and collagen type I messenger RNA (mRNA) expression levels was measured by RT-qPCR in the TPC-1 thyroid cell line after 72 h, 1 week, and 2 weeks of cell culture. (B) Representative phase contrast images for 72 h (upper panel) and 1 week (lower panel) of cell culture of TPC-1 cell line are shown in the left column. In the right column, matrix calcification was detected with Alizarin Red staining after 72 h and 1 week of cell culture. Dark brown areas correspond to ECM rich in calcium deposits.